【文獻標題】The sex steroid precursor dehydroepiandrosterone prevents nonalcoholic steatohepatitis by activating the AMPK pathway mediated by GPR30

【作者】Longlong Li , Hongjun Wang , Yao Yao , et.al

【作者單位】南京農業(yè)大學（Nanjing Agricultural University）

【文獻中引用產(chǎn)品】

小鼠雌二醇(E2)ELISA試劑盒

小鼠睪酮(T)ELISA試劑盒

小鼠環(huán)磷酸腺苷(cAMP)ELISA試劑盒

小鼠腫瘤壞死因子α(TNF-α)ELISA試劑盒

小鼠白介素6(IL-6)ELISA試劑盒

【關鍵詞】Dehydroepiandrosterone ,Biotransformation ,Nonalcoholic steatohepatitis ,AMP-Activated protein kinase ,G protein-coupled ,estrogen receptor

【DOI】10.1016/j.redox.2021.102187 【影響因子(IF)】11.79

【出版期刊】《Redox Biology》

【產(chǎn)品原文引用】

Steroid hormone (E2 and T) levels in HepG2 cells were measured by using the ELISA kits (Shanghai Hengyuan Biological Technology Co., Ltd, China), according to the manufacturer’s instructions.

Cyclic AMP (cAMP) levels in HepG2 cells and BRL-3A cells were determined by ELISA kits (Shanghai Hengyuan Biological Technology Co., Ltd, China) following the manufacturer’s instructions.

After treatment, the cell culture supernatant of HepG2 cells and BRL-3A cells was collected for testing. ELISA kits (Shanghai Hengyuan Biological Technology Co., Ltd, China) were used to determine the releases of TNF-α and IL-6.

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